Composition and action of MAD. MAD is composed from small (<10kD) dendrimers positively charged at their surface. A number of peptides, helper epitopes and stimulators of T-helper proliferation (patent pending) are covalently attached to some of the dendrimer. When the antigen is mixed with MAD, the large, positively charged complex molecule is obtained, which effectively binds to antigen receptors on dendritic cells, and effectively stimulate T-helper activity, thus inducing strong and specific immune response.
MAD (Modulating Antigenicity Dendrimers)
Not every antigen has the properties required for its optimal processing, presentation and effective immune response, therefore, various adjuvants are used to boost the antibody production. Most of them helps forming a depot of the antigen, and only some are involved in the stimulation of immune response. We have previously introduced adjuvant IMMACCEL-R, accelerating the immune response. Herewith we present new adjutant, MAD, that increases titre of specific antibodies in serum 4-30 times.
To the date MAD was intensely tested in several species of animals, and was used by our partner companies for Mighty Rabbit or Quick Mighty protocols for high titer custom serum production. In total over 1500 custom projects were performed using MAD with excellent results.
What MAD allows to achieve?
A typical example of MAD action: a low-immunogenic 25 kD fusion protein was used for rabbit vaccination with and without MAD. Serum collected at the end of the protocol from control and experimental rabbit differed in the titre 30 times.
Another example demonstrating that using MAD not only the serum has higher titre of specific IgG is achieved, but the specificity of resulting serum is higher. A 16kD fusion protein was used for rabbit immunizations. As can be seen, not only the titre of serum is much higher (left), but the serum is more specific (right). If the reaction of serum to specific antigen is 100%, then without MAD the background binding to irrelevant antigen is almost 40% of specific binding, whereas in serum obtained with MAD specific binding is higher, and non-specific - substantially lower (done for the same dilution of serum).
Substantially increases titre and affinity of specific IgGs produced by immunized animal;
Unique, patent pending technology;
Works with protein (also small size) antigens, poorly immunogenic antigens, hapten antigens, peptide antigens coupled to KLH or another carrier;
Allows to decrease amount of antigen, especially effective for low immunogenicity antigens
MAD can be used instead of Alum, or in combination with a CFA / IFA for the best result
MAD adjuvants are species-specific, and available for immunization of Rabbit or Mouse. For other species please inquire, since they are not produced on request only.
MAD adjuvants are non-toxic and do not cause strong inflammation at the site of injection
Summary of MAD features:
improve humoral response
ADJUVANT T1/T4 DATA SHEET
ADJUVANT T1/T4 MSDS
ADJUVANT T1/T4 SAFETY FOR ANIMALS INFORMATION